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Measurement of membrane fluidity and passive diffusion of S. cerevisiae WT cells induced by BA. Fluidity was determined by measuring the steady-state fluorescence polarization with DPH as the fluorescent probe as described in the legend for Fig. 2A. Diffusion was determined by measuring the extracellular R6G concentration as described in the legend for Fig. 2B. The values are means ± standard deviations (indicated by the bars) of three independent experiments.
