TABLE 1.
Primers used for PCR amplification of β-lactamase genes
| Gene | Primer | Sequence (5′→3′) | PCR product size (pb) |
|---|---|---|---|
| E. coli ampC promoter | AB1 | GATCGTTCTGCCGCTGTG | 271 |
| AmpC2 | GGGCAGCAAATGTGGAGCAA | ||
| TEM | Forward | ATGAGTATTCAACATTTCCG | 867 |
| Reverse | CTGACAGTTACCAATGCTCTC | ||
| SHV | Forward | GGTTATGCGTTATATTCGCC | 867 |
| Reverse | TTAGCGTTGCCAGTGCTC | ||
| OXA-1 | OXA-1F | ACACAATACATATCAACTTCGC | 814 |
| OXA-1R | AGTGTGTGTTTAGAATGGTGATC | ||
| OXA-2 | OXA-2F | TTCAAGCCAAAGGCACGATAG | 704 |
| OXA-2R | TCCGAGTTGACTGCCGGGTTG | ||
| OXA-10 | OXA-10F | CGTGCTTTGTAAAAGTAGCAG | 651 |
| OXA-10R | CATGATTTTGGT GGGAATGG |