TABLE 2.
Detection endpoints of 41- and 70MS-PCRs
| Codon | No. of copies per reactiona
|
||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| a/a | P-1(41L, 70K)
|
P-2(41M, 70K)
|
P-3(41M>L, 70R)c
|
||||||||||||||||||
| 166 (1×) | 16.6 (10×) | 8.3b (20×) | 4.2 (40×) | 2.1 (80×) | 1 (160×) | 0.5 (320×) | 30 (1×) | 15 (2×) | 7.5 (4×) | 3.8b (8×) | 1.9 (16×) | 0.9 (32×) | 42.8 (80×) | 21.4 (160×) | 10.7 (320×) | 5.3 (640×) | 2.7b (1,280×) | 1.3 (2,560×) | 0.7 (5,120×) | ||
| 41 | M | − | − | − | − | − | − | − | + | + | + | + | − | − | + | + | + | + | + | − | − |
| L | + | + | + | − | − | − | − | − | − | − | − | − | − | + | + | − | − | − | − | − | |
| 70 | K | + | + | + | − | − | − | − | + | + | + | + | − | − | − | − | − | − | − | − | − |
| R | − | − | − | − | − | − | − | − | − | − | − | − | − | + | + | + | + | + | − | − | |
a
“1×”, etc., indicates the number of dilutions.
b
Endpoint detection limit of the assay.
c
The 41L variant is the minor population.