Fig. 3.

Detection of truncated p53 in CRC cell lines with anti-p53 antibody DO-1. Cell extracts were analyzed by Western blotting by using DO-1 as described in Materials and Methods. Samples from 1–10 are: C70, C84, COLO678, COLO741, Gp2d, HCA7, LS513, SW948, VACO4A, and VACO429. Cell line LS513 (position 7) is a positive control from a line with normal TP53. C70 (position 1) and HCA7 (position 6) each showed two different products and SW948 (position 8) lacked evidence of any product, whereas the remaining 6 lines each showed a single product.