Table 1. PCR amplification conditions for exons 1–11 of TP53 and their corresponding DHPLC analysis conditions.

Exon	Primer sequences (5′-3′)	PCR product size, bp	PCR Tm, °C	DHPLC denaturating temperature, °C/gradient initial buffer B, %
1	cacagctctggcttgcaga	442	66	60/58, 64/52
	agcgattttcccgagctga
2	agctgtctcagacactggca	317	64*	65/47
	gagcagaaagtcagtcccatg
3-4	agacctatggaaactgtgagtgga	631	56*	N/A
	gaagcctaagggtgaagagga
5-6	cgctagtgggttgcagga	550	64	61/57, 66/49
	cactgacaaccacccttaac
7	ctgcttgccacaggtctc	283	64	58/54, 66/46
	tggatgggtagtagtatggaag
8-9	gttgggagtagatggagcct	455	64	57/57, 63/50
	ggcattttgagtgttagactg
10	ctcaggtactgtgtatatacttac	351	59	57/55, 65/46
	atactacgtggaggcaagaat
11	tcccgttgtcccagcctt	476	58	56/58, 62/53
	taacccttaactgcaagaacat

^*With the addition of 0.5 × Q (Qiagen PCR amplification kit).