TABLE 4.

Summary of kinetic parameters for the interaction of oseltamivir carboxylate with different variants of influenza N1 NA^b

Mutation	kon (M−1 s−1)	koff (s−1)	Ki (nM)a
Wild type (His274)	5.8 × 105	5.7 × 10−4	1.0 (0.3)
His274Asn	1.7 × 106	1.8 × 10−4	0.1 (0.1)
His274Gly	1.8 × 106	3.7 × 10−4	0.2 (0.2)
His274Ser	1.1 × 106	5.8 × 10−4	0.5 (0.1)
His274Gln	1.7 × 106	5.7 × 10−4	0.3 (0.5)

^aThe values in parentheses were obtained from experiments designed for direct measurement of K_i.

^bValues were means of data from three or more independent experiments. Standard deviations were less than 7% of the mean value.