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. 2002 Dec;46(12):3750–3755. doi: 10.1128/AAC.46.12.3750-3755.2002

FIG. 3.

FIG. 3.

PFGE patterns of SmaI-digested genomic DNA of the S. pyogenes strains involved in the mating between an iMLS-B isolate (iB1) used as the donor and a fusidic acid- and rifampin-resistant derivative of an iMLS-C isolate (iC4fr) used as the recipient. Lane 1, donor strain iB1; lane 2, a transconjugant selected for erythromycin (64 μg/ml), fusidic acid (10 μg/ml), and rifampin (10 μg/ml) resistance; lane 3, recipient strain iC4fr. Lambda DNA concatemers (lane λ) were used as molecular size markers. The arrow indicates a ca. 290-kb band of the transconjugant that replaced a ca. 270-kb band of the recipient.