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Inhibition of gp120-CD4 binding. A 96-well plate coated with soluble CD4 was incubated with HIV-1 IIIB gp120 in the presence or absence of compounds for 1 h at room temperature. After extensive washes the bound gp120 was detected by anti-gp120 peroxidase-conjugated antibodies. Results represent percentages of gp120 binding compared to that for untreated gp120 samples (100%).
