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Fig. 3. Phylogenetic analysis of hMPVs. (a) Complete genome (nucleotide) ML tree. The ML tree was constructed using 949 complete hMPV genomes from NCBI. MAFFT and trimAl were used for alignment, and ModelFinder and IQ-TREE in PhyloSuite for nucleotide model selection and ML tree inference. Different lineages were highlighted by different branch colours. Strain AY640317.1 (Avian pneumovirus) was used as outgroup. The 26 strains from 2020 to 2024 isolated in this study were highlighted by bars with different colour. Branch length was ignored. (b) NJ tree of amino acids for the F, SH and G genes of Ningbo strains.