Fig. 1.

Graphic of the bioprinting, hydrogel formation, and cell culture. A) Hydrogel precursor containing pentenoate-functionalized hyaluronic acid (PHA), dithiothreitol (DTT), Irgacure 2959, and PBS with cells were mixed and loaded into UV-protected cartridges. PHA precursors were bioprinted in a 2 × 4 grid of rectangular prisms (8 × 6 × 0.3 mm, L × W × H) and onto a 24 × 76 mm glass microscope slide. B) Hydrogels were crosslinked after exposure to 312 nm UV light for 2 min. C) Silicon well chambers were sealed around the hydrogels and medium was added. Cells were cultured for 7 days.