Table 2.

Therapeutic strategies for targeting Piezo1 in different functional stages.

Intervention stage	Target function	Intervention direction	Potential strategies	Expected effects
Infiltration phase	Promote migration	Activation/Upregulation	1. Pharmacological Activation: Local administration of Piezo1 agonists (e.g., Yoda1). 2. Genetic Enhancement: Utilizing CRISPRa or overexpression vectors to upregulate Piezo1.	Enhance T cell motility and improve tumor homing efficiency.
Effector phase	Reverse exhaustion	Inhibition/Degradation	1. Pharmacological Blockade: Using small molecule inhibitors (e.g., GsMTx4, Dooku1). 2. Gene Silencing: Utilizing siRNA/shRNA to knockdown Piezo1. 3. Protein Degradation: Developing Piezo1-targeted PROTAC degraders.	Alleviate mechanical inhibition, enhance cellular traction force and cytotoxic function.