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Percent recovery of L. interrogans serovar copenhageni L1-130 (L130), L. biflexa Patoc 1, S. enteritidis strain 4386 (SE 4386), and E. coli strain DH5α after penetration of polarized MDCK cell monolayers between 15 and 240 min postinfection. Each point is the mean percent recovery ± standard deviation; each assay was done in triplicate for each organism. We counted 25 squares to enumerate the organisms in the lower chamber (experiment done in triplicate).
