BIP	Backward inner primer
BLAST	Basic Local Alignment Search Tool
COI	Cytochrome c oxidase subunit I
Ct	Cycle threshold
D.	Dirofilaria
DNA	Deoxyribonucleic acid
EDTA	Ethylenediaminetetraacetic acid
FIP	Forward inner primer
FN	False negative
FP	False positive
F3	Forward outer primer
B3	Backward outer primer
LAMP	Loop-mediated isothermal amplification
LF	Loop forward primer
LB	Loop backward primer
MEGA	Molecular Evolutionary Genetics Analysis
NaCl	Sodium chloride
NaOH	Sodium hydroxide
NCBI	National Center for Biotechnology Information
ND/ND5	NADH dehydrogenase (subunit 5)
NTC	Non-template control
PCR	Polymerase chain reaction
POC	Point-of-care
qPCR	Quantitative real-time polymerase chain reaction
TN	True negative
TP	True positive