FIG. 4.

PCR analysis and sequencing of the C. parvum CpTSP8 region that contains a predicted intron. (A) C. parvum genomic DNA templates (lane 1) and reverse-transcribed RNA of C. parvum-infected HCT-8 cells (48 h p.i.; lane 2) were amplified by using sequence-specific primers spanning the 226-bp intron (Table 1), and the resulting PCR products were separated on 1.0% agarose gel. M, 100-bp DNA ladder. The arrowed band is 600 bp. (B) The RT-PCR product illustrated in lane 2 of panel A was TA cloned, and the insert of a positive colony was sequenced from both directions by using PCR primers. The functional splicing sites are indicated by arrows. Also included are 10 nucleotides of the exon flanking each side of the intron.