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. 2026 Jun 1;15(6):702. doi: 10.3390/antiox15060702
Keap1 Kelch-like ECH-associated protein 1
Nrf2 Nuclear factor-erythroid 2-related factor 2
PTM Post-translational modification
CoA Coenzyme A
Redox Reduction–oxidation
ROS Reactive oxygen species
H2O2 Hydrogen peroxide
LMW Low-molecular-weight
GSH Glutathione
BSH Bacillithiol
MSH Mycothiol
TCA Tricarboxylic acid
sMAF Small musculoaponeurotic fibrosarcoma
CNC Cap’n’collar
CsMBE CNC-sMaf binding element
Cul3
RBX1
Cullin3
RING-box protein 1
NTD N-terminal domain
BTB Broad complex–Tramtrack–Bric-a-brac
IVR Intervening region
CTD C-terminal domain
TBH tert-Butyl hydroperoxide
NEM N-ethylmaleimide
SDS Sodium dodecyl sulphate
DTT Dithiothreitol
Tris-HCl Tris hydrochloride
PIC Protease Inhibitor Cocktail
mAb Monoclonal antibody
HEK293 Human embryonic kidney 293
Pank1β Pantothenate kinase 1β
DMEM Dulbecco’s Modified Eagle Medium
FBS Foetal bovine serum
RT Room temperature
TCL Total cell lysate
WB Western blot
HA-mKeap1WT HA-tagged wild-type mouse Keap1
HA-mKeap111Cys-less HA-tagged mouse Keap1 mutant containing 11 substituted cysteine residues
SDS-PAGE SDS polyacrylamide gel electrophoresis
TBSt Tris-buffer saline containing 0.1% Tween 20