ISH	in situ hybridization
ISS	in situ sequencing
RCA	rolling circle amplification
smFISH	single-molecule fluorescence in situ hybridization
MERFISH	multiplexed error-robust fluorescence in situ hybridization
seqFISH	sequential fluorescence in situ hybridization
osmFISH	ouroboros single-molecule fluorescence in situ hybridization
STARmap	spatially resolved transcript amplicon readout mapping
ExSeq	expansion sequencing
ExFISH	expansion fluorescence in situ hybridization
EASI-FISH	expansion-assisted iterative fluorescence in situ hybridization
FFPE	formalin-fixed paraffin-embedded
HybISS	hybridization-based in situ sequencing
RAEFISH	reverse-padlock amplicon-encoding fluorescence in situ hybridization
PRISM	profiling of RNA in situ through single-round imaging
FISH	fluorescence in situ hybridization
ISTDECO	in situ transcriptomics decoding by deconvolution
QC	quality control
H&E	hematoxylin and eosin
MOSAICA	multi omic single-scan assay with integrated combinatorial analysis
SNR	signal-to-noise ratio
CARE	content-aware image restoration
JSIT	joint sparse method for imaging transcriptomics
scRNA-seq	single-cell RNA-sequencing
SPLIT	spatial purification of layered intracellular transcripts
FICTURE	factor inference of cartographic transcriptome at ultra-high resolution
OME-NGFF/OME-Zarr	open microscopy environment next-generation file formats and Zarr format
HubMAP	Human BioMolecular Atlas Program
BICCN/BICAN	BRAIN Initiative Cell Census Network and Cell Atlas Network
DAPI	4′,6-diamidino-2-phenylindole