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. 2026 May 27;16(6):785. doi: 10.3390/biom16060785
AEX Anion exchange chromatography
BMEA N-benzyl-N-methylethanolamine
CEX Cation exchange chromatography
CHO Chinese hamster ovary
DNA Deoxyribonucleic acid
HCPs Host cell proteins
HIC Hydrophobic interaction chromatography
IgG Immunoglobulin G
ISEC Inverse size-exclusion chromatography
mAb Monoclonal antibody
MMC Mixed-mode chromatography
VEGF Vascular endothelial growth factor
Aevp Integrated conductivity peak area [mL.mS/cm]
BCA Bicinchoninic acid assay
ca Equilibrium concentration of IgG aggregates [g/L]
cm Equilibrium concentration of IgG monomers [g/L]
CV Column volume [mL]
qa Equilibrium adsorbed amount of IgG aggregates [mg/g]
qm Equilibrium adsorbed amount of IgG monomers [mg/g]
Kp Distribution coefficient [-]
Λmax Measured conductivity of 10 mM HCl solution [mS/cm]
Λset Theoretical conductivity of 10 mM HCl solution [mS/cm]
mM Mass of the resin [g]
Mw Weight-average molecular weight [g/moL]
rh Hydrodynamic radius [nm]
rp Pore radius [nm]
S Selectivity coefficient [−]
ti Retention time of solute probe [min]
tmax Retention time of the largest dextran [min]
tmin Retention time of glucose [min]
tR Retention time [min]
vp Specific pore volume [mL/g]
VHCl Volume of HCL solution [mL]
Vtot Dead volume of the system [mL]
w Dry mass fraction [%]
Λ Ligand density [µmol/g]