TABLE 2.
Transmission electron microscopy results with various culture and infection conditionsa
| Evaluation parameter and cell line | Culture and infection conditions
|
|||
|---|---|---|---|---|
| DMEM | Artificial urine | DMEM + P. mirabilis | Artificial urine + P. mirabilis | |
| Crystal adhesion | ||||
| Caco-2 | − | + | − | + |
| SV-HUC-1 | − | − | − | + |
| HT29 | − | + | − | − |
| HT29-1 8N2 | − | − | − | − |
| HT29-FU | − | − | − | − |
| HT29-MTX | − | − | − | + |
| Intracytoplasmic crystal formation | ||||
| Caco-2 | − | + | − | + |
| SV-HUC-1 | − | − | − | + |
| HT29 | − | + | − | + |
| HT29-1 8N2 | − | − | − | + |
| HT29-FU | − | + | − | + |
| HT29-MTX | − | − | − | + |
| Intramitochondrial crystal formation | ||||
| Caco-2 | − | − | − | + |
| SV-HUC-1 | − | − | − | + |
| HT29 | − | − | − | − |
| HT29-1 8N2 | − | − | − | − |
| HT29-FU | − | − | − | + |
| HT29-MTX | − | − | − | + |
| Bacterial adhesion | ||||
| Caco-2 | − | − | + | + |
| SV-HUC-1 | − | − | + | + |
| HT29 | − | − | + | − |
| HT29-1 8N2 | − | − | − | + |
| HT29-FU | − | − | − | + |
| HT29-MTX | − | − | + | − |
| Bacterial invasion | ||||
| Caco-2 | − | − | − | + |
| SV-HUC-1 | − | − | + | + |
| HT29 | − | − | − | + |
| HT29-1 8N2 | − | − | − | + |
| HT29-FU | − | − | − | + |
| HT29-MTX | − | − | − | + |
a
Cell pellets and monolayer cultures were used in infected artificial urine conditions; all other conditions were evaluated in monolayer conditions (+, present; −, absent).