TABLE 3.
RASA resultsa
| Cell line | Strain | RASA results
|
|||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| DMEM
|
Amoxicillin
|
Augmentin
|
Cotrimoxazole
|
Ciprofloxacin
|
Gentamicin
|
Nitrofurantoin
|
Metronidazole
|
||||||||||
| Group | Mean (SE); Pb | Group | Mean (SE); Pb | Group | Mean (SE); Pb | Group | Mean (SE); Pb | Group | Mean (SE); Pb | Group | Mean (SE); Pb | Group | Mean (SE); Pb | Group | Mean (SE); Pb | ||
| Caco-2 | AB129 | + | 0.60 (0.51); 0.305 | − | 0.80 (0.66); 0.294 | − | 0.80 (0.58); 0.242 | + | 1.0 (0.32); 0.034* | − | −0.40 (0.68); 0.587 | − | 0.60 (0.40); 0.208 | − | 1.0 (0.63); 0.189 | + | 1.0 (0.32); 0.034* |
| AB474 | + | + | + | + | + | + | + | + | |||||||||
| AB780 | − | − | + | + | − | − | + | + | |||||||||
| AB964 | + | + | + | + | + | + | + | + | |||||||||
| ATCC 49565 | − | + | − | − | − | − | + | − | |||||||||
| SV-HUC-1 | AB129 | − | 0.62 (0.39); 0.189 | − | 0.02 (0.44); 0.966 | + | 1.42 (0.24); 0.004* | − | 0.02 (0.01); 0.178 | + | −0.18 (0.36); P=0.646 | − | 0.02 (0.01); 0.178 | − | 0.02 (0.01); 0.178 | + | 0.02 (0.01); 0.178 |
| AB474 | + | + | + | − | + | + | + | − | |||||||||
| AB780 | + | + | + | − | + | + | + | + | |||||||||
| AB964 | − | + | + | + | − | − | − | − | |||||||||
| ATCC 49565 | − | − | + | − | − | − | − | − | |||||||||
| HT29 | AB129 | + | 0.40 (0.24); 0.178 | − | 0.60 (0.24); 0.070 | − | M=0.60 Se=(0.40); P=0.208 | − | 0.60 (0.24); 0.070 | − | −0.20 (0.73); 0.799 | − | 0.20 (0.66); 0.778 | − | 0.20 (0.86); 0.828 | + | 0.80 (0.37); 0.099 |
| AB474 | + | − | + | + | + | + | + | + | |||||||||
| AB780 | − | + | + | + | + | − | + | ||||||||||
| AB964 | − | + | − | + | − | + | − | − | |||||||||
| ATCC 49565 | − | + | − | − | − | − | + | − | |||||||||
| HT29-18N2 | AB129 | − | 1.0 (0.44); 0.086 | − | 1.0 (0.44); 0.086 | − | 4.20 (0.58); 0.002* | − | 0.60 (0.39); 0.202 | − | 0.00 (0.02); 1.0 | − | 0.20 (0.19); 0.347 | − | 0.60 (0.39); 0.202 | − | 0.60 (0.67); 0.424 |
| AB474 | + | + | − | − | − | − | − | − | |||||||||
| AB780 | + | + | + | − | − | − | − | − | |||||||||
| AB964 | − | + | + | − | − | − | − | + | |||||||||
| ATCC 49565 | − | − | + | − | − | − | − | − | |||||||||
| HT29-FU | AB129 | + | 0.80 (0.37); 0.099 | − | 0.40 (0.24); 0.178 | + | 1.0 (0.55); 0.142 | + | 1.20 (0.20); 0.004* | − | −0.20 (0.49); 0.704 | − | 0.20 (1.02); 0.854 | − | 0.20 (0.86); 0.828 | + | 1.0 (0.55); 0.142 |
| AB474 | + | + | + | + | + | + | + | + | |||||||||
| AB780 | − | + | + | + | − | − | − | + | |||||||||
| AB964 | − | − | − | + | − | − | − | + | |||||||||
| ATCC 49565 | + | − | − | + | − | + | − | ||||||||||
| HT29-MTX | AB129 | + | 0.20 (0.37); 0.621 | − | 0.00 (0.55); 1.0 | − | 0.20 (0.58); 0.749 | + | 0.80 (0.20); 0.016* | −0.40 (0.68); 0.587 | − | 0.20 (0.86); 0.828 | − | −0.40 (0.75); 0.621 | + | 0.60 (0.51); 0.305 | |
| AB474 | − | + | + | + | − | + | + | − | |||||||||
| AB780 | − | + | + | + | + | + | − | + | |||||||||
| AB964 | + | − | − | + | − | − | − | + | |||||||||
| ATCC 49565 | − | − | − | − | − | − | − | − | |||||||||
The logarithmic numbers of CFU of lysed cells/logarithmic numbers of CFU in cell-free controls were divided into two groups of ≤1 (indicated by “−”) and >1 (indicated by “+”) and used in a binomial test to evaluate the possible effectivity of antibiotics. The numbers of CFU were read from MacConkey agar plates. The logarithmic colony counts were used in paired t tests with the corresponding cell-free control to assess P. mirabilis resistance to antibiotic therapy in the presence of cells. Mean, the mean of paired differences. P, P value in paired t test. *, Significant in paired t test (indicated by boldface). Boldfacing also indicates possible effective antibiotics in this cell line (binomial test, all controls are negative; samples are positive when the colony count exceeds that of the corresponding control).
The overall mean, standard error, and P value for each subgroup of data is provided.