FIG. 2.
Influence of cultivation temperature and the mammalian host environment on B. burgdorferi Bdr synthesis profiles. Cell lysates of B. burgdorferi B31MIpc cultivated at either 23 or 37°C (indicated above each lane) were analyzed by SDS-PAGE in 15% polyacrylamide gels (16 by 20 cm) and immunoblotted. In addition, host-adapted bacteria (HA) obtained from DMCs that had been implanted in rats for 96 h were also analyzed. The immunoblots were screened with either anti-BdrD (data not shown), anti-Bdr, anti-BdrE, anti-BdrF3, or anti-Fla Ab as indicated below each panel. For reference, the identities of a subset of the expressed proteins are indicated. The Fla immunoblot demonstrates that protein loadings were equal in all lanes.