FIG. 3.

Bdr production profiles in B. burgdorferi 297 rpoN and rpoS knockout mutants. B. burgdorferi 297 mutants (rpoS or rpoN mutants) (17) were cultivated in the presence of erythromycin and harvested, and the cells were lysed by sonication. The lysates were analyzed by SDS-PAGE (15% polyacrylamide gels [16 by 20 cm]) and immunoblotted. The immunoblots were screened with the anti-Bdr antiserum. The migration positions of paralogs whose production was influenced by the rpo mutations are indicated by the arrows. The migration positions of OspC and of molecular mass markers (in kilodaltons) are shown between the two gels. The antiserum employed is indicated above each panel. Abbreviations are as follows: WT, wild-type B. burgdorferi 297; S⁻, rpoS mutant; N⁻, rpoN mutant.