FIG. 6.

Demonstration of Bdr production in fed and unfed ticks through IFAs. IFAs were performed as described in Materials and Methods. IFAs were performed using unfed ticks (A and B) and fed ticks (C and D). In panels A and C, anti-Bdr antiserum (α-Bdr) was used as the primary Ab and goat anti-rabbit Ab conjugated with fluorescein was the secondary Ab, and in panels C and D, anti-Fla antiserum (α-Fla) was used as the primary Ab and goat anti-mouse Ab conjugated with rhodamine was the secondary Ab. As a negative control, IFAs were performed using preimmune sera (data not shown).