EDC	N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride
NHS	N-hydroxysuccinimide
MES	Morpholine ethanesulfonic acid monohydrate
RSC	Ready-to-eat sea cucumber
N-H group	Hydrogen bonds disruption control group
TPA	Texture properties analysis
SP	Soluble protein
BSA	Bovine serum albumin
DNPH	2,4-dinitrophenylhydrazine
FHYP	Free hydroxyproline
FAN	Free ammonia nitrogen
DSC	Differential scanning calorimetry
FTIR	Fourier transform infrared spectroscopy
CD	Circular dichroism
SEM	Scanning electron microscope
VG	Van Gieson
ANOVA	Analysis of variance