TABLE 3.
pH, lactobacillus, and/or H2O2 concn | Effecta of bovine catalase concnb in overlay agar on inhibition
|
||||
---|---|---|---|---|---|
0 U | 0.5 U | 5 U | 50 U | 500 U | |
Base agar pH 7.4 | |||||
L. crispatus | + | + | − | − | − |
L. jensenii | + | + | − | − | − |
18 mM H2O2 | + | − | − | − | − |
50 mM H2O2 | + | + | − | − | − |
100 mM H2O2 | + | + | − | − | − |
Base agar pH 5.8 | |||||
L. acidophilus | + | + | + | + | − |
L. crispatus | + | + | + | + | − |
L. jensenii | + | + | + | − | − |
L. gasseri | + | + | + | − | − |
18 mM H2O2 | + | − | − | − | − |
50 mM H2O2 | + | + | − | − | − |
100 mM H2O2 | + | + | − | − | − |
Symbols: +, inhibition; −, absence of inhibition.
Catalase concentration is expressed in units per mg of protein (1 U decomposes 1 μmol of H2O2 per min at 25°C and pH 7.0).