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Western blotting with MAb 7H6 of CaMP or total cell extracts and secreted material of acapsular (7698) and encapsulated (6995) C. neoformans. Western blot analysis with MAb 7H6 was performed on total cell extracts and secreted materials as described in Materials and Methods. Yeasts were cultured in RPMI at 30°C for 72 h. Total protein loaded amounts were ∼10 μg/lane for all samples. The molecular mass markers in kilodaltons are shown at the left.
