FIG. 5.
(A) Survival of mice after intravenous (iv) challenge with a lethal dose of viable C. albicans. Mice were treated with saline or 10 μg of CnMP in saline 24 and 6 h before challenge. (Inset) Survival of mice immunized with C. albicans materials and challenged with 2 × 105 C. albicans cells. The results are reported as median survival time (MST) and as number of dead animals over total number of animals tested (D/T). ∗, P < 0.05 (CnMP- or CaMP-treated versus saline-treated mice). (B) Effect of CnMP on viable C. albicans cells in the kidneys. Mice were treated with saline or 10 μg of CnMP 24 and 6 h before challenge with C. albicans (2.5 × 105 cells/mouse). At different times, mice were killed and viable yeast cells in the kidneys (CFU) were counted by plating samples of homogenized tissue on Sabouraud agar. Data are means ± standard deviations for four or five mice. ∗, P < 0.05 (CnMP-treated versus saline-treated mice).