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Detergent purification of P1. Proteins solubilized in Empigen or SDS by consecutive detergent extraction at increasing temperatures were separated by SDS-PAGE on a 12.5% acrylamide gel and silver stained. Lane 1, Empigen extraction at 37°C; lane 2, Empigen extraction at 60°C; lane 3, SDS extraction at 100°C; lane 4, molecular weight marker; lane 5, starting material.
