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Detection of a second C3-binding protein in an MtbΔhbhA23 cell lysate by C3 ligand affinity blotting analysis. Cell lysates of M. tuberculosis H37Rv (lane 1) and MtbΔhbhA23 (lane 2) were either stained with Coomassie blue R-250 (A) or transferred to polyvinylidene difluoride membrane and subjected to the C3 ligand affinity blotting technique, using NHS as the source of C3 (B). Molecular mass markers are indicated on the left.
