TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Characteristics | Reference or source |
|---|---|---|
| E. coli strains | ||
| ORN103 | thr-1 leu-6 thi-1 Δ(argF-lac) U169 xyl-7 ara-13 mtl-2 gal-6 rpsL tonA2 fhuA2 minA monB recA13 Δ(fimABCDEFGH) | 33 |
| XL-1 Blue | supE44 hsdR17 recA1 endA1 gyrA46 thi relA1 lac F′ [proAB+lacIqlacZ ΔM15 Tn10] (Tetr) | Stratagene |
| EH41 | Wild-type EHEC O113:H21 HUS isolate (Smr) | This study |
| EH41lpfA− | lpfA deletion mutant of EH41 (Smr Kmr) | This study |
| 15-26 | EH41 pstS::TnphoA (Smr Kmr) | This study |
| 83/39 | Wild-type REPEC O15:H− | 2 |
| Plasmids | ||
| PCR-Script | High-copy-number cloning vector (Ampr) | Stratagene |
| pWSK29 | Low-copy-number cloning vector (Ampr) | 35 |
| pWSK:lpf | pWSK29 carrying lpfABCDO113 | This study |
| pRT733 | Suicide plasmid carrying TnphoA (Ampr) | 32 |
| pKD46 | Low-copy-number thermosensitive plasmid encoding red recombinase | 7 |
| pKD4 | Template for PCR amplification of kanamycin cassette for red recombinase-mediated recombination | 7 |