FIG. 1.
Schematic of the strategy used to mutagenize JSG435 (PmrAc) with MudJ in order to obtain mutants in PmrA-regulated genes and/or genes involved in PM resistance. The MudJ transposon encodes genes involved in β-galactosidase production, as well as a kanamycin resistance cassette. The SalI site used in cloning the MudJ-chromosomal DNA junction for sequencing is shown. Black circles represent blue colonies resulting from active fusion of lacZ in the MudJ transposon to an ORF. White circles symbolize Kanr colonies that contain inactive fusions. Light grey colonies represent a light blue phenotype corresponding to low-level transcription of lacZ. Abbreviations: Kan, kanamycin; Tet, tetracycline; Cam, chloramphenicol; XG, X-Gal.