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. 1988 Apr;53(4):493–496. doi: 10.1016/S0006-3495(88)83128-X

Branching Photocycle of Sensory Rhodopsin in Halobacterium Halobium

Hiroyuki Ohtani, Takayoshi Kobayashi, Motoyuki Tsuda
PMCID: PMC1330222  PMID: 19431721

Abstract

Temperature effect of the photocyle of sensory rhodopsin (sR) was studied by nanosecond spectroscopy. Though the formation yield of sRM (sR370) was sharply decreased with temperature, those of sRK (sR680) and sRL were insensitive to temperature changes. These results show the existence of the branching process back to sR from sRL. The absorption maxima for sRK and sRL were 595 ± 5 and 555 ± 15 nm, respectively.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bogomolni R. A., Spudich J. L. Identification of a third rhodopsin-like pigment in phototactic Halobacterium halobium. Proc Natl Acad Sci U S A. 1982 Oct;79(20):6250–6254. doi: 10.1073/pnas.79.20.6250. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Hazemoto N., Kamo N., Terayama Y., Kobatake Y., Tsuda M. Photochemistry of two rhodopsinlike pigments in bacteriorhodopsin-free mutant of Halobacterium halobium. Biophys J. 1983 Oct;44(1):59–64. doi: 10.1016/S0006-3495(83)84277-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hildebrand E., Dencher N. Two photosystems controlling behavioural responses of Halobacterium halobium. Nature. 1975 Sep 4;257(5521):46–48. doi: 10.1038/257046a0. [DOI] [PubMed] [Google Scholar]
  4. Kalisky O., Ottolenghi M., Honig B., Korenstein R. Environmental effects on formation and photoreaction of the M412 photoproduct of bacteriorhodopsin: implications for the mechanism of proton pumping. Biochemistry. 1981 Feb 3;20(3):649–655. doi: 10.1021/bi00506a031. [DOI] [PubMed] [Google Scholar]
  5. Korenstein R., Sherman W. V., Caplan S. R. Kinetic isotope effects in the photochemical cycle of bacteriorhodopsin. Biophys Struct Mech. 1976 Dec 22;2(3):267–276. doi: 10.1007/BF00535372. [DOI] [PubMed] [Google Scholar]
  6. Polland H. J., Franz M. A., Zinth W., Kaiser W., Kölling E., Oesterhelt D. Early picosecond events in the photocycle of bacteriorhodopsin. Biophys J. 1986 Mar;49(3):651–662. doi: 10.1016/S0006-3495(86)83692-X. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Spudich E. N., Spudich J. L. Control of transmembrane ion fluxes to select halorhodopsin-deficient and other energy-transduction mutants of Halobacterium halobium. Proc Natl Acad Sci U S A. 1982 Jul;79(14):4308–4312. doi: 10.1073/pnas.79.14.4308. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Spudich J. L., Bogomolni R. A. Mechanism of colour discrimination by a bacterial sensory rhodopsin. Nature. 1984 Dec 6;312(5994):509–513. doi: 10.1038/312509a0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Spudich J. L., Bogomolni R. A. Spectroscopic discrimination of the three rhodopsinlike pigments in Halobacterium halobium membranes. Biophys J. 1983 Aug;43(2):243–246. doi: 10.1016/S0006-3495(83)84345-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Takahashi T., Mochizuki Y., Kamo N., Kobatake Y. Evidence that the long-lifetime photointermediate of s-rhodopsin is a receptor for negative phototaxis in Halobacterium halobium. Biochem Biophys Res Commun. 1985 Feb 28;127(1):99–105. doi: 10.1016/s0006-291x(85)80131-5. [DOI] [PubMed] [Google Scholar]
  11. Takahashi T., Watanabe M., Kamo N., Kobatake Y. Negative phototaxis from blue light and the role of third rhodopsinlike pigment in halobacterium cutirubrum. Biophys J. 1985 Aug;48(2):235–240. doi: 10.1016/S0006-3495(85)83776-0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Tsuda M., Govindjee R., Ebrey T. G. Effects of pressure and temperature on the M412 intermediate of the bacteriorhodopsin photocycle. Implications for the phase transition of the purple membrane. Biophys J. 1983 Nov;44(2):249–254. doi: 10.1016/S0006-3495(83)84296-9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Tsuda M., Hazemoto N., Kondo M., Kamo N., Kobatake Y., Terayama Y. Two photocycles in halobacterium halobium that lacks bacteriorhodopsin. Biochem Biophys Res Commun. 1982 Oct 15;108(3):970–976. doi: 10.1016/0006-291x(82)92094-0. [DOI] [PubMed] [Google Scholar]
  14. Tsuda M., Nelson B., Chang C. H., Govindjee R., Ebrey T. G. Characterization of the chromophore of the third rhodopsin-like pigment of Halobacterium halobium and its photoproduct. Biophys J. 1985 May;47(5):721–724. doi: 10.1016/S0006-3495(85)83969-2. [DOI] [PMC free article] [PubMed] [Google Scholar]

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