FIG. 1.
Reactivity of MAb MEST-1 with promastigotes of Leishmania spp. by solid-phase RIA. (A) Preparation containing 2 × 106 parasites that was serially diluted, adsorbed onto 96-well plates, and incubated with MEST-1. Symbols: •, L. major; ◊, L. braziliensis; ▵, L. chagasi; ×, L. amazonensis. (B) Preparation containing 1 × 106 L. major promastigotes adsorbed onto 96-well plates that was delipidated (○) or not delipidatd (•) with IHW (55:20:25) and incubated with MEST-1, as described in Materials and Methods. (Inset) MEST-1 reactivity with IHW fraction removed from wells and adsorbed on a new 96-well plate.