FIG. 3.

CTL responses are markedly enhanced by tucaresol. Groups of HLA-A2/K^b transgenic mice were immunized twice with either P3M.65, P3M.65 γ, or P3M.65, T. Splenocytes were cultured with the HLA-A2 binding peptide Mhsp65 (9.369) for 5 to 6 days and thereafter were used as effectors in conventional ⁵¹Cr release assays using as target cells Jk-A2/kb cells pulsed with either Mhsp65 (9.369) (M.65 pep), an irrelevant influenza virus peptide (Infl. pep), or medium, as described in Materials and Methods. Mice were injected twice with 20 μg of pDNA per injection as described in Materials and Methods. Mice in the group treated with tucaresol received 1 mg of tucaresol s.c. simultaneously. The experiment was repeated with similar results.