TABLE 1.

Effects of tucaresol and cytokine-encoding plasmids on specific antibody responses

Immunization	IgG			IgG2a			IgG1
	Responsea	P relative tob:		Response	P relative to:		Response	P relative to:
		P3	P3M.65		P3	P3M.65		P3	P3M.65
P3	0.23			0.05			0.08
P3M.65	0.42	<0.05		0.54	<0.001		0.32	<0.001
P3M.65, T	0.67	<0.001	<0.01	0.97	<0.001	<0.001	0.42	<0.001	>0.05
P3M.65 G	0.61	<0.001	<0.05	0.70	<0.001	>0.05	0.54	<0.01	>0.05
P3M.65 γ	0.28	>0.05	<0.05	0.25	<0.05	<0.01	0.08	>0.05	<0.001
P3M.65 G γ	0.39	<0.05	>0.05	0.90	<0.01	>0.05	0.52	<0.05	>0.05

^aEach response value is a mean based on the optical densities at 405 nm of individually tested, 1:100-diluted serum samples from six experiments using four to six mice per group. Mice were injected twice with 20 μg of pDNA per injection as described in Materials and Methods. Sera were collected and tested for the presence of Mhsp65-specific antibodies by using isotype-specific ELISA.

^bValues in each immunization group are compared to those obtained in the P3 and P3M.65 immunization groups. Statistical significance was calculated as P values by using Student's t test.