TABLE 3.
Recovery of S. enterica serotypes Dublin, Gallinarum, and Abortusovis from selected enteric and systemic sites 24 h after oral inoculation of calvesa
| Tissue or material | Bacterial recovery (log10 CFU/ml ± SEM)
|
||
|---|---|---|---|
| SD3246 | SG9 | SAO44 | |
| Liver | E (5/5) | E (2/5) | E (0/3) |
| HLN | E (5/5) | E (0/5) | E (0/3) |
| Lung | E (4/5) | E (2/5) | E (0/3) |
| MDLN | E (2/5) | E (0/5) | E (0/3) |
| Spleen | E (5/5) | E (2/5) | E (0/3) |
| Intestinal contents | |||
| Ileum | 4.53 ± 0.61 | 2.88 ± 0.25 | E (2/3) |
| Cecum | 4.79 ± 0.42 | 3.01 ± 0.28 | E (2/3) |
| Colon | 5.14 ± 0.45 | 3.14 ± 0.27 | E (2/3) |
| Intestinal mucosa | |||
| Ileum | 5.00 ± 0.43 | 3.87 ± 0.28 | 3.02 ± 0.36 |
| Cecum | 5.21 ± 0.21 | 3.89 ± 0.15 | 3.51 ± 0.76 |
| Colon | 3.89 ± 0.26 | 2.51 ± 0.21 | 2.57 ± 1.29 |
| Mesenteric lymph nodes | |||
| Ileum | 4.67 ± 0.27 | 4.2 ± 0.03 | 3.28 ± 0.42 |
| Cecum | 4.78 ± 0.2 | 4.13 ± 0.1 | 3.25 ± 0.64 |
| Colon | 4.5 ± 0.3 | 2.87 ± 0.39 | 3.26 ± 0.66 |
a
Triplicate samples were taken from each tissue or material, and means were calculated to give a value per animal. Each figure represents the mean of five calves each for serotypes Dublin (SD3246) and Gallinarum (SG9) and three calves for serotype Abortusovis (SAO44) with the SEM. The limit of accurate quantification was 2.0 log10 CFU/g, and samples that contained salmonellae in numbers too low to quantify are indicated by E (positive on enrichment culture) with the number of positive tissues/number of calves used parentheses. HLN, hepatic lymph node; MDLN, mediastinal lymph node.