TABLE 2.

Antibody titers following immunization with PpAMA1 FVO or PpAMA1 3D7

Immunogena and rabbit	ELISA titerb to:		FVO/3D7 titer ratio
PpAMA1 FVO	PpAMA1 3D7
PpAMA1 FVO
85506	43,250	21,060	2.05
85508	53,650	24,210	2.22
85511	88,550	42,850	2.07
85514	32,240	17,440	1.85
85516	36,400	15,360	2.37
Geometric mean	47,473.2	22,566.7	2.10
PpAMA1 3D7
85507	6,330	16,670	0.38
85509	38,400	83,650	0.46
85510	18,390	37,850	0.49
85515	42,250	78,400	0.54
85517	26,770	52,650	0.51
Geometric mean	21,915.9	46,519.6	0.47

^aRabbits were immunized three times at 3-week intervals, each time intramuscularly with 50 μg of antigen emulsified in Montanide ISA720 (days 0, 21, and 42). Titers shown are from bleeds 2 weeks after the third immunization (day 56).

^bELISA titers presented measure total IgG to the indicated recombinant protein in arbitrary antibody units (see Materials and Methods).