TABLE 4.

Antibody titers following immunization of rabbits with PpAMA1 FVO, PpAMA1 3D7, or a combination of the two antigens^a

Immunogen and rabbit	ELISA titer to		FVO/3D7 titer ratio
	PpAMA1 FVO	PpAMA1 3D7
PpAMA1 FVO
STO1	107,931	42,643	2.53
STO2	64,139	27,739	2.31
STO3	87,124	35,602	2.45
STO4	167,085	59,528	2.81
Geometric mean	100,192.7	39,790.9	2.52
PpAMA1 3D7
STO5	38,271	98,540	0.39
STO6	37,534	94,441	0.40
STO7	45,403	122,013	0.37
STO8	29,815	65,086	0.46
Geometric mean	37,342.5	92,718.6	0.40
PpAMA1 FVO plus     PpAMA1 3D7
STO17	73,346	78,890	0.93
STO18	62,356	58,240	1.07
STO19	248,275	264,520	0.94
STO20	65,517	80,499	0.81
Geometric mean	92,872.1	99,454.2	0.93

^aRabbits were immunized twice, 4 weeks apart (days 0 and 28), each time intramuscularly with 25 μg of antigen emulsified in Montanide ISA720 (either 25 μg of PpAMA1 FVO, 25 μg of PpAMA1 3D7, or 12.5 μg of PpAMA1 FVO plus 12.5 μg of PpAMA1 3D7). Titers shown are from bleeds 2 weeks after the second immunization (day 42).