TABLE 2.

Ability of C. jejuni to translocate across T84 polarized cells is independent of invasive potential

C. jejuni strain	No. of bacteria
	In basolateral medium				Cell associateda (4 h)	Internalizedb (7 h)
	0-1 h	1-2 h	2-3 h	3-4 h
81-176	(1.1 ± 0.1) × 106	(1.4 ± 0.1) × 106	(1.4 ± 0.2) × 106	(1.1 ± 0.2) × 106	(2.2 ± 0.7) × 106	(4.5 ± 0.5) × 104
F38011	(1.3 ± 0.2) × 106	(1.1 ± 0.1) × 106	(9.0 ± 0.8) × 105	(8.8 ± 0.5) × 105	(1.1 ± 0.3) × 106	(3.7 ± 0.4) × 104
ciaB mutant	(1.5 ± 0.3) × 106	(1.3 ± 0.8) × 106	(2.0 ± 0.9) × 106	(1.9 ± 0.5) × 106	(1.2 ± 0.3) × 106	(5.1 ± 0.2) × 103c
ciaB mutant plus pMEK100	(2.3 ± 0.3) × 106	(1.9 ± 0.3) × 106	(2.3 ± 0.3) × 106	(2.5 ± 0.1) × 106	(1.2 ± 0.2) × 106	(1.2 ± 0.1) × 104

^aC. jejuni-inoculated T84 cells were rinsed three times in PBS after a 4-h incubation period. The number of cell-associated (adherent) bacteria was determined as outlined in Materials and Methods.

^bC. jejuni-inoculated T84 cells were rinsed three times in Entero-STIM after 4 h of incubation, followed by 3 h of incubation with gentamicin to kill extracellular bacteria as outlined in Materials and Methods.

^cMean value is significantly different (P < 0.01) from that obtained with the C. jejuni wild-type isolate and C. jejuni ciaB mutant harboring pMEK100.