TABLE 7.
C. jejuni association with Fn localized to the basolateral surface of T84 polarized cells maximizes bacterial adherence and invasion
| Type of C. jejuni F38011 cells | % of adherent and internalized bacteriaa
|
||
|---|---|---|---|
| Anti-Fn | EGTA | EGTA + anti-Fn | |
| Adherent | 97 ± 9 | 185 ± 15b | 35 ± 3c |
| Internalized | 108 ± 16 | 367 ± 21b | 67 ± 13c |
a
Assays were performed as outlined in Materials and Methods. Results are presented as the percent adherent and internalized bacteria relative to the values (100%) obtained for the wild-type isolate alone.
b
Value significantly different (P < 0.01) from that obtained from the inoculation of T84 cells with the C. jejuni F38011 wild-type isolate.
c
Value significantly different (P < 0.01) from that obtained from inoculation of the EGTA-treated T84 cells with the C. jejuni F38011 wild-type isolate.