FIG. 1.

(A) P. falciparum (NF54) CS protein showing the B-cell epitope (NANP)n within the major repeat region (bars), the T1 epitope within the 5′ minor repeat region (stippled), and the universal T∗ epitope in the C terminus (black). (B) The synthetic dsDNA fragment encoding the malaria epitope inserted in each plasmid is shown. Amino acid insertions contributed by the restriction sites are underlined, and the stop codons are indicated by asterisks. (C) Hybrid HBc protein showing the CS T1 and B repeat epitopes (hatched box) inserted between HBc amino acids 78 and 79, a region located at the tip of the core particle surface spikes (3, 8), and the T∗ epitope fused to amino acid Val₁₄₉ at the C terminus of the truncated HBc protein (solid box).