TABLE 1.
Numbers of recombinant phage binding to fibronectin and proteolytic fragments of fibronectin
| Ligand | No. of bound phagea |
|---|---|
| Whole fibronectin | 2.43 × 105 (1.35 × 105−3.24 × 105) |
| 30-kDa N-terminal fragment | 4.5 × 102 (2.7 × 102−5.4 × 102) |
| 45-kDa α-chymotrypic fragment | 0 |
| 120-kDa α-chymotrypic fragment | 6.3 × 102 (5.4 × 102−8.1 × 102) |
a
The number of bound phage CFU were determined as CFU infecting E. coli TG1 and plating the infected cells on nutrient broth no. 2 agar plates containing ampicillin. The values are the means for triplicate wells of one representative experiment of at least three experiments. The values in parentheses are the ranges of the numbers of phage recovered from the triplicate wells.