FIG. 2.

Photomicrographs showing the kinetics of infection of the trophoblastic cell populations from placentas of C. callosus and fetal tissues. (a) Rupture of a giant trophoblastic cell (T) by T. gondii (asterisk) after the 15th dop and infection. Bar, 11 μm. (b) Presence of T. gondii (arrow) in the region of labyrinth after the 16th dop. Bar, 11 μm. (c) Presence of T. gondii (arrow) in a spongiotrophoblast at the 19th dop. Bar, 11 μm. (d) T. gondii inside a giant trophoblastic cell at the 20th dop, showing that the parasite was unstained by PAS (arrow). Bar, 11 μm. (e) Electromicrograph from a fetal blood vessel in the labyrinth region at the 20th dop, showing the presence of T. gondii (asterisk) inside a reticulocyte (r). Bar, 1.30 μm. (f) Fetal nervous tissue containing T. gondii stained by alkaline phosphatase substrate in the immunohistochemistry assay (arrow) at the 16th dop. Bar, 11 μm. (g) Fetal liver containing T. gondii inside a hepatocyte stained by alkaline phosphatase substrate in the immunohistochemistry assay (arrow). Bar, 48 μm.