TABLE 1.
Serovar Typhimurium strains and plasmids used in this study
| Strain | Plasmid-encoded proteinsa | Reference |
|---|---|---|
| SB824(pHR231) | SycE, YopE1-138/LLO51-363/M45 | 31 |
| SB824(pHR241) | SycE, YopE1-138/p60130-477/M45 | 31 |
| SB824(pHR251) | SycE, YopE1-138/LLO51-363/M45, YopE1-138/p60130-477/M45 | This study |
a
Hybrid yopE gene fusions were cloned into the low-copy-number vector pWSK29. Large C-terminal portions of LLO or p60 were fused to the N-terminal 138 amino acids of YopE, resulting in hybrid proteins that contain the secretion and translocation domain of YopE. All chimeric proteins were M45 tagged at their C terminus.