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Binding of H2 polypeptide to microtiter plate wells coated with Fn (•), Fg (▴), or BSA (▪). Increasing concentrations of H2 were incubated in the wells for 1 h at room temperature. Bound protein was detected with anti-H2 antibodies and anti-rabbit AP-conjugated antibodies. Values represent the means of triplicate wells. H2 polypeptide binds immobilized Fn, but not Fg or BSA.
