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. 1974 Nov;243(1):225–242. doi: 10.1113/jphysiol.1974.sp010751

Protein and glucose-induced changes in sodium transport across the pig small intestine

C Henriques de Jesus, M W Smith
PMCID: PMC1330698  PMID: 4141370

Abstract

1. The effect of glucose and of bovine γ-globulin on short-circuit current and sodium transport was measured using new-born pig ileum before and after suckling.

2. Glucose increased the short-circuit current with an apparent Km of 1·9 and 19·5 mM for new-born unsuckled and suckled intestines respectively. The Vmax was about 80 μA cm-2 in each case.

3. Bovine γ-globulin applied to new-born pig ileum caused a 20% increase in sodium chloride influx without change of short-circuit current. The tissue conductance was also greater in the presence of globulin. These effects were not seen in ileum taken from suckled pigs.

4. p-Chloromercuriphenyl sulphonic acid (PCMPS) inhibited the globulin induced increase in sodium influx in new-born pig ileum without changing the measured short-circuit current. 0·1 mM-PCMPS also changed the characteristics of the glucose-dependent increase in short-circuit current, so that this response came to resemble that seen in ileum taken from suckled pigs. Dithioerythritol reversed this effect of PCMPS.

5. The suckling-dependent difference in results is explained in terms of a change in microvillar function taking place as a consequence of long-term pinocytosis in vivo. The ability of PCMPS to simulate some of these changes suggests that sulphydryl groups might be important regulators of microvillar membrane stability.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baintner K., Jr The physiological role of colostral trypsin inhibitor: experiments with piglets and kittens. Acta Vet Acad Sci Hung. 1973 May;23(3):247–260. [PubMed] [Google Scholar]
  2. Boulpaep E. L. Permeability changes of the proximal tubule of Necturus during saline loading. Am J Physiol. 1972 Mar;222(3):517–531. doi: 10.1152/ajplegacy.1972.222.3.517. [DOI] [PubMed] [Google Scholar]
  3. Cornell R., Walker W. A., Isselbacher K. J. Small intestinal absorption of horseradish peroxidase. A cytochemical study. Lab Invest. 1971 Jul;25(1):42–48. [PubMed] [Google Scholar]
  4. Hardy R. N., Hockaday A. R., Tapp R. L. Observations on the structure of the small intestine in foetal, neo-natal and suckling pigs. Philos Trans R Soc Lond B Biol Sci. 1971 Feb 11;259(834):517–531. doi: 10.1098/rstb.1971.0002. [DOI] [PubMed] [Google Scholar]
  5. Hardy R. N. The absorption of polyvinyl pyrrolidone by the new-born pig intestine. J Physiol. 1969 Oct;204(3):633–651. doi: 10.1113/jphysiol.1969.sp008936. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hardy R. N. The break-down of 131-I-gamma-globulin in the digestive tract of the new-born pig. J Physiol. 1969 Nov;205(2):435–451. doi: 10.1113/jphysiol.1969.sp008976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Henriques de Jesus C., Smith M. W. Sodium transport by the small intestine of new-born and suckling pigs. J Physiol. 1974 Nov;243(1):211–224. doi: 10.1113/jphysiol.1974.sp010750. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Munn E. A., Smith M. W. Proceedings: Uptake of albumin by neonatal pig ileum incubated in vitro. J Physiol. 1974 Oct;242(2):30P–32P. [PubMed] [Google Scholar]
  9. Nellans H. N., Frizzell R. A., Schultz S. G. Coupled sodium-chloride influx across the brush border of rabbit ileum. Am J Physiol. 1973 Aug;225(2):467–475. doi: 10.1152/ajplegacy.1973.225.2.467. [DOI] [PubMed] [Google Scholar]
  10. Pierce A. E., Smith M. W. The intestinal absorption of pig and bovine immune lactoglobulin and human serum albumin by the new-born pig. J Physiol. 1967 May;190(1):1–18. doi: 10.1113/jphysiol.1967.sp008189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Schaeffer J. F., Preston R. L., Curran P. F. Inhibition of amino acid transport in rabbit intestine by p-chloromercuriphenyl sulfonic acid. J Gen Physiol. 1973 Aug;62(2):131–146. doi: 10.1085/jgp.62.2.131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Schultz S. G., Curran P. F. Coupled transport of sodium and organic solutes. Physiol Rev. 1970 Oct;50(4):637–718. doi: 10.1152/physrev.1970.50.4.637. [DOI] [PubMed] [Google Scholar]
  13. Walker W. A., Cornell R., Davenport L. M., Isselbacher K. J. Macromolecular absorption. Mechanism of horseradish peroxidase uptake and transport in adult and neonatal rat intestine. J Cell Biol. 1972 Aug;54(2):195–205. doi: 10.1083/jcb.54.2.195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Warshaw A. L., Walker W. A., Cornell R., Isselbacher K. J. Small intestinal permeability to macromolecules. Transmission of horseradish peroxidase into mesenteric lymph and portal blood. Lab Invest. 1971 Dec;25(6):675–684. [PubMed] [Google Scholar]

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