TABLE 1.

Primers and amplicon sizes for loci included in the PCR assay

Locus	Primer designation	Nucleotide sequence of primer	Amplicon size(s) (bp)
bfpA	bfpA-F11	5′-GTCTGCGTCTGATTCCAATA-3′	408, 414
	bfpA-R1	5′-TCAGCAGGAGTAATAGC-3′
toxB	toxB.911F	5′-ATACCTACCTGCTCTGGATTGA-3′	602
	toxB.1468R	5′-TTCTTACCTGATCTGATGCAGC-3′
stx1	1A.251F	5′-GGGATAGATCCAGAGGAAGG-3′	624
	1A.832R	5′-CCGGACACATAGAAGGAAACTC-3′
stx2	2A.506F	5′-CTGGCGTTAATGGAGTTCAG-3′	383
	2A.848R	5′-CCTGTCGCCAGTTATCTGAC-3′
eae	eae-F1	5′-ACTCCGATTCCTCTGGTGAC-3′	389
	eae-R3	5′-TCTTGTGCGCTTTGGCTT-3′
terC	terC.106F	5′-TATGCACCGTGATGACAAGC-3′	210
	terC.275R	5′-GGCGAACCAGGAGAAGATTG-3′
ureA	ureA.109F	5′-TAACTATCCCGAATCCGTGG-3′	146
	ureA.213R	5′-GGGATCATTTCTGGTATGCCT-3′
iha	iha.138F	5′-ACGCAGCCGCCAGTGTT-3′	186
	iha.282R	5′-CCATCAATCAGTATCAGCGTGTAA-3′
invG	invG.481F	5′-TGACCTGGTCGTTAATGCTG-3′	133
	invG.572R	5′-CGCCACGTAACTCATAAGTCC-3′
spaP	spaP.143F	5′-GGACTTCAGCAAGTGCCATC-3′	173
	spaP.275R	5′-ACCACTCATGCCTGTCTCAA-3′
Z4326	ShET.1137F	5′-TCATGCAGACGCAATAAAGG-3′	125
	ShET.1221R	5′-GATACGCAAGAAGTAATCCTGG-3′
fyuA	fyuA.924F	5′-GCAGCAGCAGCATTATTCG-3′	495
mdh	mdh.269F	5′-GGTATGGATCGTTCCGACCT-3′	304
	mdh.530R	5′-GGCAGAATGGTAACACCAGAGT-3′