TABLE 2.
Oligonucleotide primers used in this studya
| Name | Sequence (5′-3′) |
|---|---|
| 1721Rb | TTAAAGACATGTCCGTCAATCC |
| SpeL_Fb | ATGAGAATTTTTTTACACCAG |
| SpeL_Fcutb | ACCATGGGAAGAGACTATTAATATTAAGG |
| SpeL_Rb | CGAATTCATTTTCTTTGTTTGTGAAT |
| 117b | AAATGGCGTCATTTTCTCA |
| SpeM_Fb | ATGTTAGGAGAAAAAATG |
| SpeM_Fcutb | ACCATGGTTTTCAGATGCTGTGTTGG |
| SpeM_Rb | CGGATCCATTTTTAGAAAAATCTTCG |
| SpeL_8169b | ACATATTAACCAAACGACAATTC |
| SpeM_8952b | AGTTATATCTAGCAATGTTTCTCCA |
| Param147b | CCAGCGCAAGAAAGACG |
| Param112b | ACATATTAACCAAACGACAATTC |
| Param134b | AGTTATATCTAGCAATGTTTCTCCA |
| 1220Lb | CAGCACCTTCCTCTTTCTCG |
| 1220Rb | GGAAAAAGAGGGACGCAAG |
| G1295Lb | GGATGAGTGAATAAATCGGTAAAC |
| G5-358D2b | CTTTTGACTGATAGTGACAAGCTG |
| SBBP4004.2b | CTTGTGTGTGTATCGCTTGCTC |
| 1237_Revb | TCATGCGGCAACACATA |
| SpeL_5TQb,c | TCGATGTTTTTGGTCTTGCATT |
| SpeL_3TQb,c | CCGGCTCCTTTTCTCTCATTAA |
| SpeL_probec | 6FAM-AAAGATCAAATACATTCAATCAATGGTGGTCTCGTTAAA-TAMRA |
| SpeM_5TQb,c | AAAGATGTAATTAATAGAACCAATATGAAGATAACA |
| SpeM_3TQb,c | AATTATCATCGTCCCAGACTCTAGTTTT |
| SpeM_probec | 6FAM-AGAAAATTGGCACCCAGTTAATATTTAACACGAATGA-TAMRA |
| SpeB_5TQc | CGCACTAAACCCTTCAGCTCTT |
| SpeB_3TQc | ACAGCACTTTGGTAACCGTTGA |
| SpeB_probec | 6FAM-GCCTGCGCCGCCACCAGTA-TAMRA |
| GyrA_5TQc | CGACTTGTCTGAACGCCAAA |
| GyrA_3TQc | TTATCACGTTCCAAACCAGTCAA |
| GyrA_probec | 6FAM-CGACGCAAACGCATATCCAAAATAGCTTG-TAMRA |
a
Primers were designed based on the genome sequence of GAS strain MGAS8232 (serotype M18) (33).
b
Primers used for sequencing the speL-speM locus.
c
Primers and probes used for real time RT-PCR analysis (TaqMan assays). Probes were labeled at the 5′ end with the reporter dye 6-carboxyfluorescein (6FAM) and at the 3′ end with the quencher 6-carboxy-N,N,N′,N′-tetramethylrhodamine (TAMRA).