TABLE 4.

P. multocida genes previously shown to respond to low iron levels in vitro (31) that were up-regulated during growth within infected chickens

Gene	Predicted function	Expression valuea
		c1	c2	c3
aroA	3-Phosphoshikimate 1-carboxyvinyltransferase	0.03	0.84	1.15
cydD	Transport ATP-binding protein	−0.01	0.64	1.58
fecB	Iron(III) dicitrate-binding periplasmic protein	−0.13	0.10	1.51
fnr	Fumarate (and nitrate) reduction regulatory protein	−0.99	1.45	1.21
folB	Dihydroneopterin aldolase	0.45	0.82	0.80
hugZ_1	Heme utilization protein	−0.25	0.96	2.11
hugZ_2	Heme utilization protein	−0.25	0.96	2.11
ilvM	Acetolactate synthase small subunit	0.14	0.80	1.71
lldD	l-Lactate dehydrogenase	−0.37	2.22	2.08
nagB	Glucosamine-6-phosphate isomerase	0.20	0.80	0.79
nrdB	Ribonucleoside diphosphate reductase, beta chain	0.60	0.46	0.85
PM0298	Unknown	−0.30	0.60	0.96
PM0300	Putative TonB-dependent receptor	−0.22	0.68	1.14
PM0336	Putative TonB-dependent receptor	0.36	1.09	2.20
PM0424	Unknown	−0.90	−0.04	0.91
PM0452	Unknown	−0.21	1.04	1.67
PM0741	Hemoglobin receptor precursor	0.08	1.02	1.20
PM0803	Unknown	−0.61	0.70	1.86
PM1171	Unknown	−0.49	0.04	1.03
PM1460	Phosphoglycerate transport system activator protein	1.26	1.23	1.29
PM1604	Unknown	0.24	−0.09	1.10
PM1727	Unknown	2.29	0.21	−0.46
pqqL	Zinc protease	−0.24	0.73	2.00
tal	Transaldolase	0.18	0.67	1.44
trpG	Anthranilate synthase component II	1.39	0.20	0.87
yfeB	Iron(III) dicitrate transport ATP-binding protein	0.10	0.91	1.70
yfeC	Iron (chelated) ABC transporter	0.02	0.35	1.07
yfeD	Iron (chelated) transporter, permease protein	0.05	0.54	1.13

^aExpression values are expressed as the log₂ of (average experimental intensity/average control intensity). Therefore, identical experimental and control expression values would be 0.0.