TABLE 2.

Oxygen-independent killing of leptospires^a

Subcellular fraction	Mean bacterial count (105 MPN) ± SD (n = 3) forb:
	Patoc1	Hardjoprajitno
Control (buffer)	17.0 ± 2.5	18.5 ± 5.3
Cytosol	16.5 ± 2.6	25.2 ± 2.0
Low-density membranes	19.1 ± 3.0	26.4 ± 3.2
High-density membranes	18.2 ± 0.7	25.2 ± 1.4
Secondary granules	15.2 ± 0.7	14.5 ± 6.0
Primary granules	4.6 ± 1.5	5.5 ± 2.0

^aLeptospira killing assays were performed by using 20 μg of protein from either cytosolic fractions or acid extracts from membrane and granule fractions. Control assays using the same amount of acid buffer were performed simultaneously. Viability was determined as described in Table 1, footnote a.

^bMPN, most probable number.