TABLE 1.
Primers used to amplify crmA and the entire gapA operon, the probe for the Southern hybridization, and the gentamicin resistance gene
| Primer | Sequencea (direction) | Gentamicin resistance gene |
|---|---|---|
| SG322 | 5′ GGGGGATCCAGACCAAACTTCCCTAAC 3′ (forward) | gapA |
| SG721 | 5′ GGGGGATCCCCTTATCGTAGAGAAGGGAGGT 3′ (reverse) | crmA |
| SG768 | 5′ AAGGGGATCCGCTCCAGCACCAACTAAGAAAATTGA 3′ (forward) | crmA |
| SG804 | 5′ GGCAATTATGATCATCTTAGGA 3′ (forward) | 3′ crmA |
| SG805 | 5′ TAGAGAAGGGAGGTTATTTT 3′ (reverse) | 3′ crmA |
| SG643 | 5′ ACACAGGAGTCTGGACTTGACTGA 3′ (forward) | GmRb |
| SG665 | 5′ TTACACAGGAGTCTGGACTTGACTCA 3′ (reverse) | GmR |
a
BamHI restriction endonuclease sites are underlined.
b
Gentamicin resistance gene from the Tn4001mod transposon.